Which are the types of chromatography according to mobile phase used?
Table of Contents
Which are the types of chromatography according to mobile phase used?
Different types of chromatography
| Technique | Stationary phase | Mobile phase |
|---|---|---|
| *Paper chromatography | solid (cellulose) | liquid |
| *Thin layer chromatography (TLC) | solid (silica or alumina) | liquid |
| *Liquid column chromatography | solid (silica or alumina) | liquid |
| Size exclusion chromatography | solid (microporous beads of silica) | liquid |
What is the mobile phase of chromatography?
Chromatography relies on two different ‘phases’: the mobile phase is the solvent that moves through the paper, carrying different substances with it. the stationary phase is contained on the paper and does not move through it.
How many types of mobile phases are there?
… total of nine different mobile phases were used ( Table 1). The composition of the mobile phases consisted of a combination of pH 2.0, 3.0 or 6.5 (higher pH values were not used due to the nature of the stationary phase) and a percentage acetonitrile composition of 20, 30 or 40\%. …
What are types of chromatography?
Types of chromatography
- Column chromatography.
- Ion-exchange chromatography.
- Gel-permeation (molecular sieve) chromatography.
- Affinity chromatography.
- Paper chromatography.
- Thin-layer chromatography.
- Gas chromatography.
- Dye-ligand chromatography.
What is the mobile phase in thin layer chromatography?
The mobile phase is a solvent chosen according to the properties of the components in the mixture. The principle of TLC is the distribution of a compound between a solid fixed phase (the thin layer) applied to a glass or plastic plate and a liquid mobile phase (eluting solvent) that is moving over the solid phase.
How many types of mobile phase are there in HPLC?
The solvents used in the HPLC mobile phase are chosen on the basis of the type of HPLC selected for the purpose. There are two types of such HPLC executed in the adsorbent column.
What are three other types of chromatography?
The chromatography techniques are: 1. Paper Chromatography 2. Thin Layer Chromatography and 3. Column Chromatography.
What type of chromatography is thin layer chromatography?
Thin layer chromatography (TLC) is an affinity-based method used to separate compounds in a mixture. In TLC, the stationary phase is a thin adsorbent material layer, usually silica gel or aluminum oxide, coated onto an inert plate surface, typically glass, plastic, or aluminum.
What are the mobile and stationary phases in chromatography?
In paper chromatography the stationary phase is water, which is supported by the paper. The paper used for chromatography contains 22\% by weight of water which is stationary phase. The mobile phase is generally a polar organic solvent and water, as both mobile and stationary phase are water paper chromatography is called liquid chromatography.
What is the mobile phase’s purpose in chromatography?
Mobile phase in chromatography is a compound used to separate components in a mixture. Most importantly, this phase can move along with the components. Consequently, the mobile phase runs through the stationary phase along with the sample.
What is the mobile phase that can I use for HPLC?
The mobile phase used in HPLC are two types and they are isocratic type and the other is gradient type. In isocratic type the mobile phase has a single solvent or more than one solvent yet the ratio of composition is fixed through out the HPLC analysis.
What is the definition of mobile phase?
The mobile phase is part of gas chromatography mass spectrometery testing. It is an an inert gas that the sample is injected into that will carry it through the stationary phase, it moves the sample so that it is mobile.