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How is a proton gradient created across the inner mitochondrial membrane during?

How is a proton gradient created across the inner mitochondrial membrane during?

At the inner mitochondrial membrane, a high energy electron is passed along an electron transport chain. The energy released pumps hydrogen out of the matrix space. The gradient created by this drives hydrogen back through the membrane, through ATP synthase.

What generates a proton gradient across the membrane?

The device that makes this possible is a large membrane-bound enzyme called ATP synthase. This enzymes creates a hydrophilic pathway across the inner mitochondrial membrane that allows protons to follow down their electrochemical gradient.

What creates the electrochemical gradient across the inner mitochondrial membrane?

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4 Calcium transport through the inner mitochondrial membrane Respiratory chain activity generates an electrochemical gradient across the IMM (− 180 mV inside) which drives the synthesis of ATP and allows Ca2 + uptake by mitochondria (Rizzuto et al., 2012; Stock et al., 1999).

How is the proton gradient maintained?

as the inner mitochondrial membrane is impermeable to protons, H+ ions cannot pass back through the inner mitochondrial membrane, into the matrix, therefore there is a higher concentration of H+ ions in the inter-membrane space, thus setting up and maintaining a proton gradient across the inner mitochondrial membrane.

How the proton gradient created across inner mitochondrial membrane is linked with the synthesis of ATP?

How is the proton gradient created?

A proton gradient is formed by two quinol (4H+4e−) oxidations at the Qo site to form one quinol (2H+2e−) at the Qi site (in total six protons are translocated: two protons reduce quinone to quinol and four protons are released from two ubiquinol molecules).

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How is a proton H +) gradient created during the ETC and how does it contribute to the formation of ATP?

The electron transport chain. The electron transport chain is a collection of membrane-embedded proteins and organic molecules, most of them organized into four large complexes labeled I to IV. In eukaryotes, many copies of these molecules are found in the inner mitochondrial membrane.

Why does proton gradient is very important across the membrane?

Across thylakoid membranes, the light induced -proton gradient is essential for ATP synthesis. As a result of proton pumping into the thylakoid lumen, an alkaline stromal pH develops, which is required for full activation of pH-dependent Calvin Benson cycle enzymes.

What is proton gradient in mitochondria?

The electrochemical proton gradient is a difference hydrogen ion concentration across a membrane producing a concentration gradient and an electrical potential gradient. The energy released by electrons flowing through this transport chain, pumps hydrogen out of the matrix space across the inner mitochondrial membrane.

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What maintains the proton gradient in the mitochondria in the absence of o2?

Oxygen also binds with free protons in the matrix to form water – removing matrix protons maintains the hydrogen gradient. In the absence of oxygen, hydrogen carriers cannot transfer energised electrons to the chain and ATP production is halted.

How is the proton gradient formed in photosynthesis?

When the light energy is absorbed by the excited electrons are passed on to the chain of electron carriers present within thylakoid membrane. This energy is utilised to pump protons from the the thylakoid membrane into the thylakoid space which creates the proton gradient.

How is a proton gradient created?