How do you calculate 10 times dilution?

For example: if you needed 10 mL of the 1:10 dilution, then you would mix 1mL of the 1M NaCl with 9mL of water. Or: if you needed 100mL of the 1:10 dilution, then you would mix 10mL of the 1M NaCl with 90mL of water.

What does it mean to dilute something 10 times?

The idea here is that in a 10 -fold dilution, the volume of the diluted solution is 10 times higher than the volume of the stock solution. This is equivalent to saying that the concentration of the stock solution is 10 times lower than the concentration of the diluted solution.

How do you make a 1/10 serial dilution?

The dilution factor or the dilution is the initial volume divided by the final volume. For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF=ViVf = 1mL10mL=110 . This is a 1:10 dilution.

Why is serial dilution usually performed with a power of 10 dilution?

Objectives of Serial Dilution Serial dilutions are commonly performed to avoid having to pipette very small volumes (1-10 µl) to make a dilution of a solution.

How do you make a 10 fold dilution with the final volume of 10mL?

To do a 10 fold dilution of 1M Stock solution to 100mL, Mix 10mL of 1M stock with 90mL of Diluent. Label the tube as Dilution 1. From Dilution 1 tube take 10mL and mix 90mL of diluent and Label it as Dilution 2.

How do you make a 10mm solution?

take 700 uL from the solution (your org comp dissolved in water) and pour it in a separate vial (2 mL) and add 300 uL of water in it. Now you have 1 mL of your stock with a molarity of 10 mM. you can use this link to calculate in future.

What is a 10 fold?

Definition of tenfold 1 : being 10 times as great or as many. 2 : having 10 units or members.

How do you dilute 10m to 1M?

Take 1 part of your stock solution and add 9 parts of solvent (usually water but sometimes alcohol or other organic solvent). In all cases you are diluting by the same factor. The concentration of the resulting solution is 1M /10 = 0.1M where 10 is the dilution factor.

How much diluent should be used in order to create a 1/10 dilution using a sample volume of 5 ml?

Answer: 1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml – 2 ml = 8 ml diluent.

How do you do a 20x dilution?

A 20-fold dilution just means the final solution is 20 times less concentrated than the original. An easy way to perform this is to take say 5 mL of your original acid using a pipette, transfer it to a 100 mL volumetric flask and then fill to the mark with distilled or RO water.

How do I make a 10 mg/mL stock solution?

To prepare a concentration of 10 µg/ml, pipette out 10 µl of the drug in a test tube using a micropipette. Then dilute it with 990 µl (making a total volume of 1 ml) of the solvent (ethanol, methanol, water etc) you are going to use in your experiment.

How do you convert mM to mg mL?

So, to convert mg/mL to mM, I divided the concentration in mg/mL (20 mg/mL) by the molecular weight of the sample (232,278 g/mole) and multiplied by 1,000.

How do you make a 10 fold serial dilution?

How do you perform a 10 fold serial dilution? The first step in making a serial dilution is to take a known volume (usually 1ml) of stock and place it into a known volume of distilled water (usually 9ml). This produces 10ml of the dilute solution. This dilute solution has 1ml of extract /10ml, producing a 10-fold dilution.

What does it mean to dilute by a factor 10?

This is equivalent to saying that the concentration of the stock solution is 10 times lower than the concentration of the diluted solution. In other words, you’re performing a 1:10 dilution, i.e. you’re diluting the stock solution by a factor fo 10.

What is a 20-fold dilution of acid?

A 20-fold dilution just means the final solution is 20 times less concentrated than the original. An easy way to perform this is to take say 5 mL of your original acid using a pipette, transfer it to a 100 mL volumetric flask and then fill to the mark with distilled or RO water.

How do you find the dilution factor of serial dilution?

Plug your dilution factor into the equation: D t = 10 x 10 x 10 x 10 = 10,000 The final dilution factor of the fourth tube in your serial dilution is 1:10,000. The concentration of your substance is now 10,000 times less than the original undiluted solution. 2