What happens if the solvent is above the start line?

The start line above the solvent level allows the solvent to move past the start line, carrying the dissolved samples along with it. The solvent will rise up the TLC plate by capillary action.

What would happen if your initial solvent level is above the baseline?

Experiment 5: Thin Layer Chromatography and Melting Point 62 4) What would happen if your solvent level is above the level of the initial spots? Answer: The spots would dissolve into the reservoir of eluting solvent.

What would happen if the solvent level is above the start line at the beginning of the experiment?

The start line above the solvent level allows the solvent to move past the start line, carrying the dissolved samples along with it.

Why does the spot have to be above the solvent level?

The solvent level has to be below the starting line of the TLC, otherwise the spots will dissolve away. Non-polar solvents will force non-polar compounds to the top of the plate, because the compounds dissolve well and do not interact with the polar stationary phase.

Why is it essential for the spots on the origination line to be above the level of the solvent when placed into the developing chamber?

It is extremely important that the solvent in the developing chamber be lower than the spotos on the plate, because the solvent must be drawn upward through the sample in order to draw the sample along with it. If the sample is dipped in to the solvent, the sample may simply dissolve in the developing solvent.

Why must the spot applied to a chromatography plate be above the level of the developing solvent?

Used to separate volatile components of a mixture with low B.P. Why must the spot be applied to the TLC plate above the level of development solvent? Low B.P. and thus more likely to evaporate off the plate before separation can occur.

Why should the spot in chromatography be small?

In paper chromatography, a starting line in drawn on the paper in pencil (pencil so that it does not dissolve in the solvent and affect the results). Small spots of each sample are placed on the starting line. If a substance is more strongly attracted to the paper than the solvent then it will move a short distance.

Why must the spots on the paper not be allowed to touch the solvent?

The reason is that the plate will be immersed in the eluting solvent to effect the separation, and the eluting solvent should not touch the spots when the separation begins.

Why must the spot applied to the chromatography paper be above the level of the developing solvent?

What is the spot in chromatography?

Spotting consists of using a micro pipet to transfer a small amount of this dilute solution to one end of a TLC plate, in this case a thin layer of powdered silica gel that has been coated onto a plastic sheet. The spotting solvent quickly evaporates and leaves behind a small spot of the material.

Why must the spots applied to a chromatography plate paper be above the level of the developing solvent?

What happens in paper chromatography?

Chromatography is a method of separating mixtures by using a moving solvent on filter paper. The solvent flows along the paper through the spots and on, carrying the substances from the spot. Each of these will, if the solvent mixture has been well chosen, move at a different rate from the others.

Why must the solvent level be below the spots in chromatography?

If you placed it below the level of the solvent in the beaker, the solvent would just flow at a much faster rate and instead of separating the components in the spot, they would simply be washed away. Why must the solvent level be below the colored spots in chromatography?

Why must the start line be above the solvent level?

In paper chromatography, why must the start line be above the solvent level? The start line above the solvent level allows the solvent to move past the start line, carrying the dissolved samples along with it.

Why is the solvent level below the origin of the ink?

Having solvent levels below the origin give a more accurate spread of colours, as the water is allowed to begin properly soaking and ink is not lost in the solution upon direct contact. Also, there is little to no motion through the paper below the solvent level and hence, the ink/dye won’t be able to travel up the chromatography paper.

Why must food dye place on starting line above the solvent?

So that you can measure how far it traveled. Why must food dye place on starting line above the solvent in chromatography experiment? Having solvent levels below the origin give a more accurate spread of colours, as the water is allowed to begin properly soaking and ink is not lost in the solution upon direct contact.